Skip to content

Input file name collision #121

@vella12-osu

Description

@vella12-osu

Description of the bug

Hello,

I am running several samples using spatialvi in different directories and they are all throwing the same error. Hope you can help:

I run spatialvi as follows:

nextflow run nf-core/spatialvi -r dev -profile singularity --outdir ./ --input ./sample.sheet.csv --spaceranger_probeset path/to/probe/set/Visium_Human_Transcriptome_Probe_Set_v2.0_GRCh38-2020-A.csv

My sample sheet looks like this:

sample,fastq_dir,cytaimage,slide,area
sample name,path/to/fastq/dirs/sample name,path/to/img/dir/image name,slide ID,area

The error message is as follows:

ERROR ~ Error executing process > 'NFCORE_SPATIALVI:SPATIALVI:READ_DATA (1)'

Caused by:
Process NFCORE_SPATIALVI:SPATIALVI:READ_DATA input file name collision -- There are multiple input files for each of the following file names: sample name/scalefactors_json.json, sample name/raw_feature_bc_matrix.h5, sample name/tissue_hires_image.png, sample name/tissue_lowres_image.png

Container:
/path/to/running/dir/work/singularity/docker.io-erikfas-spatialvi.img

Tip: you can try to figure out what's wrong by changing to the process work dir and showing the script file named .command.sh

-- Check '.nextflow.log' file for details
ERROR ~ Pipeline failed. Please refer to troubleshooting docs: https://nf-co.re/docs/usage/troubleshooting

-- Check '.nextflow.log' file for details

Command used and terminal output

Relevant files

No response

System information

No response

Metadata

Metadata

Assignees

Labels

bugSomething isn't working

Type

No type

Projects

No projects

Milestone

No milestone

Relationships

None yet

Development

No branches or pull requests

Issue actions