@@ -5,9 +5,9 @@ cd wtdbg2 && make
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# assemble PacBio reads
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./wtdbg2 -t 16 -i pacbio.fa.gz -fo prefix -L 5000
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# assemble Nanopore reads
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- ./wtdbg2 -t 16 -i ont.fa.gz -fo prefix
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+ ./wtdbg2 -t 16 -i ont.fa.gz -fo prefix -L 5000
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# derive consensus
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- ./wtpoa-cns -t 16 -i prefix.ctg.lay > prefix.ctg.lay.fa
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+ ./wtpoa-cns -t 16 -i prefix.ctg.lay -fo prefix.ctg.lay.fa
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```
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## <a name =" intro " ></a >Introduction
@@ -41,7 +41,7 @@ produces the final consensus in FASTA. A typical workflow looks like this:
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./wtpoa-cns -t 16 -i prefix.ctg.lay -fo prefix.ctg.lay.fa
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```
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where ` -t ` specifies the number of CPU cores (` -t 0 ` to use all processors). When the default doesn't work
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- well, you may need to apply more options.
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+ well, you may need to apply more options. See [ README-ori.md ] [ readme-ori ] for more help.
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Wtdbg2 combines normal k-mers and homopolymer-compressed (HPC) k-mers to find
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read overlaps. Option ` -k ` specifies the length of normal k-mers, while ` -p `
@@ -58,7 +58,7 @@ the assembly step (not including the consensus step):
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| Dataset | Genome| Coverage | Wtdbg2 options| CPU hours| Peak RAM|
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| :-----------------------| -----:| ---------:| :-------------| --------:| -------:|
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| [ E. coli] [ pbcr ] | 4.6Mb | PacBio x20| | | |
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- | [ C. elegans] [ ce ] | 100Mb | PacBio x80| | | |
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+ | [ C. elegans] [ ce ] | 100Mb | PacBio x80| -L5000 -e4 | 3.3 | 9.7G |
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| [ Human CHM1] [ chm1 ] | 3Gb | PacBio x60| -L5000 -e4 | 378.5 | 252.7G|
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| [ Human NA12878] [ na12878 ] | 3Gb | ONT x30 | -S2 -e2 | 197.4 | 244.9G|
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| [ Axolotl] [ axosra ] | 32Gb | PacBio x32| -L5000 -AS2 | 3189.7 | 1593.6G|
@@ -76,6 +76,7 @@ the assembly step (not including the consensus step):
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Please use the [ GitHub's Issues page] [ issue ] if you have questions. You may
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also directly contact Jue Ruan at
[email protected] .
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+ [ readme-ori ] : https://github.com/ruanjue/wtdbg2/README-ori.md
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[ miniasm ] : https://github.com/lh3/miniasm
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[ canu ] : https://github.com/marbl/canu
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[ falcon ] : https://github.com/PacificBiosciences/FALCON
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